Solution Information | help | |
Enzyme: | Large T antigen | |
inhibitor: | BDBM59059 | |
substrate: | n/a | |
Solution Type: | Aqueous | |
pH at Preparation: | n/a | |
Temp. Prep.: | n/a | |
Comments: | Protocol: Cytotoxicity was evaluated using Madin Darby Canine Kidney Cells (MDCK, ATCC CCL-34). This assay was conducted with 999 of the 1205 compounds designated as active in the confirmation and counterscreens. Compounds were plated in dose response format (in less than 1% DMSO final concentration), followed by the addition of cells. Cells were grown and incubated with compounds in DMEM/pen/strep/L-glutamine/10% FBS, for 72 hr at 37 degrees C/5% CO2. At the end of the incubation time cells were assayed for viability using Cell Titer GLO (Promega). Compounds that were considered cytotoxic resulted in a decreased luminescent signal. Compounds that interfere with luciferase will appear as toxic in this screen. | |
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